Check out the most popular applied biosystems products for genetic analysis. A total touch screen user interface for preprogramming and guided user manuals. This document is believed to be complete and accurate at the time of publication. Audience this guide is intended for novice and experienced geneamp pcr system 9700. Wear powderfree gloves when you handle the halogen lamp. Important indicates information that is necessary for proper instrument operation. A realfast and real versatile approach to realtime pcr. Powerplexr y23 system for use on the applied biosystems.
Abi prism applied biosystems 7000 sequence detection system. Applied biosystems abi 7500 real time pcr video youtube. Applied biosystems recommends that you perform specific instrument maintenance tasks according to the schedule outlined in the table below. Applied biosystems 9800 fast thermal cycler information sheet the applied biosystems 9800 fast thermal cycler is an instrument used in the lab to amplify segments of dna, using the principles of the polymerase chain reaction pcr. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applicatio. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. Document part number description applied biosystems 7500 fast dx realtime pcr instrument user guide for the security, auditing, and esignature module in sds software v1. The applied biosystems geneamp pcr system 9700 autolid dual 96 sample block module and dual 96 sample block module user guide provides the information you need to operate the dual 96 sample block module with the automatic or manual lid. For the applied biosystems 7500 real time pcr system and the stratagene mx3000p, the use of rox reference dye lmp is recommended. Applied biosystems 7900ht fast realtime pcr system and. Applied biosystems 75007500 fast realtime pcr system getting started guide for presenceabsence. For additional documentation, see how to obtain support on page 6. Abi prism 7000 sequence detection systems relative. Preface how to obtain support 6 applied biosystems 7500 fast dx realtime pcr instrument reference guide examples of the user attention words appear below.
Documentation user attention words five user attention words appear in the text of all applied biosystems user documentation. Each word implies a particular level of observation or action as described below. Abi prism 7000 sequence detection system and applied biosystems 7500 realtime pcr system 6 user bulletin how to view component dye signals to view the component dye signals from a quantifilerkit run, select the component subtab within the results view of the sds software. General import formatsapplied biosystemsbioradcorbett researchroche agilent. Quantifiler kits user manual pn 4344790d nfstc projects. The 7900ht instrument is optimized for use with applied biosystems. Abi prism 7000 sequence detection systems spectral calibration kit. Tb green advantage qpcr premix user manual 051019 takara bio usa, inc.
This manual is written for principal investigators and laboratory staff who conduct absolute quantification assays using the applied biosystems 730075007500 fast realtime pcr system. Please refer to the sequence detection system user guide, chapter 8 pure dye assay. Information in this document is subject to change without notice. The use of rox reference dye lsr or lmp is optional. Detection system user guide, the abi prism 7700 sequence detection system user s manual, the geneamp 5700 sequence detection system user s manual, the applied biosystems 73007500 re al time pcr system relative quantitation getting started guide, the applied biosystems 73007500 real time pcr system.
Applied biosystems 7000 sequence detection system the abi prism 7000 sequence detection system is a realtime pcr system that detects and quantitates nucleic acid sequences. View online or download applied biosystems 7500 fast installation and maintenance manual, getting started. Applied biosystem 7900ht high speed realtime pcr system user manual and 7500 high speed realtime pcr system this manual is written by major researchers and guides to start a. Applied biosystems 7000, 7500, or 7500 fast realtime pcr system life technologies applied biosystems steponeplus realtime pcr system life technologies lightcycler roche 0. Applied biosystems 7500 real time pcr user manual the applied biosystems 7500 fast realtime pcr system offers maximum individual user logins can be added for up to applied biosystems 7500 fast realtime pcr system we supply a dell notebook or tower computer with the 7500 fast system. The instrument consists of two main components, a sample block and a heating cover. Applied biosystems 7500 fast and 7500 realtime pcr systems. Twister users guide part number 66503 from zymark corporation. Applied biosystems 7000, 7500, or 7500 fast realtime pcr system life technologies. Email promega technical services if you have questions on use of this system. For more details about how to do this, refer to the instrument user manual supplied by. Note provides information that may be of interest or help but is not critical to the use.
Applied biosystems assumes no responsibility for any errors that may appear in this document. Expression of cox2 mrnas was quantified by qpcr using an abi prism sequence detection system applied biosystems, foster city, ca, usa. The quantifiler kits users manual provides information about and. If you are viewing this page as a nonregistered user, the prices displayed is list price. Technical specifications this applied biosystem prism 7000 sequence detection system is in excellent condition. Abi prism 7000 sequence detection system and sds software v1. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Abi prism 7900 sequence detection system thermo fisher. Follow specific safety practices when using this instrument. Applied biosystem 7900ht high speed realtime pcr system user manual and 7500 high speed realtime pcr system this manual is. Documents document view page applied biosystems 2720. Before starting the run, the instrument may pause up to 10 minutes to allow the heated cover to reach the correct temperature.
The kit must be used only as an ivd and be handled by qualified technicians who are trained in techniques of molecular biology applied to diagnostics. Materials to be supplied by the user plexor calibration kit, set a cat. Licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, or the licensing department, roche molecular systems, inc. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Applied biosystems 73007500 fast realtime pcr systems chemistry guide, 2005. Abi prism 7900ht sequence detection system user guide. For use with the abi prism 7000, 7700, and 7900ht sequence detection. Introduction the tb green advantage qpcr premix is a reagent supplied at 2x concentration, consisting of a fulllength taq polymerase with a hotstart taq antibody and tb green dye, that has been specially designed for realtime pcr using the dye intercalator method. Applied biosystems 5700 applied biosystems 7000 applied biosystems 7300 applied biosystems 7700 applied biosystems 7900 applied biosystems 7900 ht fast applied biosystems 7900ht. In no event shall applied biosystems be liable for incidental, special, multiple, or. Two user attention words appear in applied biosystems user documentation. Applied biosystems 7000 sequence detection system rtpcr andbio. It is desirable to select an instrument with user friendly software that performs the most desirable functions and has flexibility in terms of data output so that it can easily be manipulated in downstream statistical analysis software packages. This manual is believed to be complete and accurate at the time of publication.
See user s manual or package insert for limited label license and trademark information. Familiarity with microsoftwindows2000 operating system. Role of xenobiotic metabolizing cytochrome p450s article snippet. Audience this guide is intended for novice and experienced veriti thermal cycler users who use the instrument to perform pcr. Applied biosystems 7900ht fast realtime pcr system user bulletin. To verify your client connection, you need a valid user id. Set up, run, and analyze experiments using the 75007500 fast system. Reagents and disposables a complete line of reagents including taqman fast universal pcr master mix, taqman universal master mixes, sybr green i master mixes, and disposables including 96well plates, is available for use with the applied biosystems 7500 and 7500 fast realtime. This manual is written for principal investigators and laboratory staff who conduct relative quantification studies fo r gene expression using the abi prism7000 sequence detection system 7000 sds instrument. See user s manual or package insert for limited label license. Quantifiler kits user s manual ix safety safety alert words four safety alert words appear in applied biosystems user documentation at points in the document where you need to be aware of relevant hazards.
The abi prism 7000 sequence detection system can be used to determine the absolute quantity of a target nucleic acid sequence in a test sample by analyzing the cycletocycle change in fluorescence signal as a result of amplification during pcr. Applied biosystems 75007500 fast thermo fisher scientific. Applied biosystems 730075007500 fast realtime pcr system installation and maintenance guide, revision e. Plexor hy system for the applied biosystems 7500 and 7500.
User guide or the applied biosystems 73007500 realtime pcr. Jun 14, 2019 33xl getting started guide by applied biosystems, revision d 062010. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Free of magnesium ion and separate mgcl 2 solution is provided. Visit the web site to verify that you are using the most current version of this technical manual. Each alert wordimportant, caution, warning, dangerimplies a particular level of observation or action, as defined below. Applied biosystems 7500 fast 7500 fast dx realtime pcr system.
Tb green advantage qpcr premix user manual takara bio. The magmax express 24well low throughput platform is the premier automated platform to meet your throughput needs. The abi prism 7000 sequence detection system qpcr rtpcr 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection with fluorescentbased pcr chemistries. This products resides on a fisher scientific gsa or va contract. Magmax express magnetic particle processor brbasic. Applied biosystems 7500 fast dx realtime pcr instrument. For further information contact the director of licensing, applied biosystems, 850 lincoln. Applied biosystems 7900ht fast realtime pcr system and sds. It seamlessly incorporates the rapid, reliable, and cost efficient magnetic beadbased extraction of nucleic acids that you expect from magmax technology.
Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Available in several convenient sizes with separate mgcl 2 solution for versatility. An assay reagent containing premixed primers and a viclabeled probe applied biosystems. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. The applied biosystems 7500 fast dx realtime pcr instrument, with sds v1.
The applied biosystems 7500 and 7500 fast realtime pcr systems are covered by us patent no. Detection on the abi prism 7000 sequence detection system. The applied biosystems veriti user guide provides information about installing, using and maintaining the applied biosystems veriti thermal cycler. For further information contact the director of licensing, applied biosystems, 850 lincoln centre drive, foster city, california 94404, usa. Applied biosystems 2720 thermal cycler user manual.
Applied biosystems 7500 applied biosystems 7500 fast applied biosystems viia 7 stratagene mx3000p stratagene mx3005p stratagene mx4000 compatible instruments. Abi prism 7000 sequence detection system fisher scientific. Use the table of contents in the left navigation pane to find your topic of interest, or enter. To contact applied biosystems technical support, use the telephone or fax numbers. Complete preparation helps ensure a smooth installation process, as well as correct and safe instrument operation. Powerplexr y23 system for use on the applied biosystemsr. Applied biosystems 7000 applied biosystems 7300 applied biosystems 7700. The following user attention words appear in applied biosystems user documentation.
Applied biosystems 7500 fast and 7500 realtime pcr. Applied biosystems 7000 sequence detection system rtpcr. Applied biosystems abi applied biosystems pcr applied biosystems 2720 thermal cycler tags. System installation and maintenance guide for more detailed maintenance procedures. Preparing reagents and buffers in the qiaamp dsp virus kit handbook. The kit is designed and optimized for use with the abi prism 7000. The 7000 combines thermal cycling, fluorescence detection, and. Quantifiler duo dna quantification kit user s manual pn. Refer to the abi prism 7000 sequence detection system user guide or the appliedbiosystems 73007500 realtime pcr system installation and maintenance guide for more detailed. In no event shall applied biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this manual or the. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Applied biosystems 75007500 fast realtime pcr system getting started guide for genotyping experiments v preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems 75007500 fast realtime pcr system 75007500 fast system.
Import this reaction data to the applied biosystems 7500 realtime pcr system and then start the run. Applied biosystems 75007500 fast realtime pcr software v2. The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Abi prism 7000 system and applied biosystems 7500 system user. The abi prism 7000 sequence detection system 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection. In realtime pcr, cyclebycycle detection of accumulated pcr product is possible by combining thermal cycling, fluorescence detection, and application specific software. Quantifiler human and y human male dna quantification kits. Abi prism 7900 sequence detection system thermo fisher bioz.
Abi prism applied biosystems 7000 sequence detection. Reviews andbio has been a great partner for our used equipment needs and always provide timely updates of new arrivals of consigned equipment. Before using the kit read the user manual carefully and completely. The applied biosystems 7500 is todays top choice in rt pcr. Applied biosystems abi prism 377 dna sequencer reuzeit. Applied biosystems 7000 sequence detection system grizzly. Geneamp pcr system 9700 user s manual, revision h 062010. Applied biosystems abi 7000 sequence detection system sold for parts documentation. Applied biosystems perkin elmer abi prism 310 genetic analyzer. The abi prism 3100 genetic analyzer includes patented technology licensed from hitachi, ltd. Provided mgcl 2 solution offers versatility for optimizing pcr and maximum pcr performance diluted 1. Abi prism 7000 sequence detection system user guide. Veriti thermal cycler user guide vii preface how to use this guide purpose of this guide the applied biosystems veriti user guide provides information about installing, using and maintaining the applied biosystems veriti thermal cycler.
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